| Popis: |
Combining several one-dimensional miniaturized separation techniques in series has the potential to be an exceptionally comprehensive analysis approach. Sample species can be separated and identified based on a battery of characteristics not available from any single technique. µIEF is attractive as a first dimension of such a multi-dimensional system due to the equilibrium nature of the final separation, rapid separation capabilities (1min), concentrating qualities (100×), and high resolution (ΔpI = 0.005). As a step towards integration of µIEF with a second dimension (e.g., miniaturized capillary electrophoresis), this work investigates the robustness of µIEF as an initial dimension and explores means to reliably extract a purified, concentrated sample zone from a complete µIEF assay. |
