| Popis: |
Percutaneous absorption of 14C-2-mercaptobenzothiazole (14C-2-MBT) was studied by applying it to intact and abraded skin of guinea pig. Of the dose 6.14% and 8.39% were excreted to urine of intact skin animals and 27.42% and 34.58% were of abraded skin animals for 24 and 48 hr after application, respectively. At 1 hr after a subcutaneous (sc) injection of 14C-2-MBT, high radioactive concentration was found in the kidney, thyroid and liver in this order. At 6 hr after injection, the highest distribution in kidney, next higher in thyroid and blood, and no distribution in brain were observed. At 15 min after sc injection, the greatest concentration was observed in the excretory organs such as gallbladder, kidney, bladder and lachrymal gland by autoradiography. The main excretion route was urine and 64% and 92% of dose were excreted in 1 and 6 hr after sc injection, respectively. Two metabolites, glucuronide and sulfate of 2-MBT, were identified in urine by thin-layer chromatography, and 7.62% of 2-MBT and 90.00% of conjugates were determined in the sampled urine during 6 hr after sc injection by isotope dilution method. |
