Soft PEG-Hydrogels with Independently Tunable Stiffness and RGDS-Content for Cell Adhesion Studies
| Autor: | Addie H. Kusters, Jan C. M. van Hest, S.A. Bode, Dennis W. P. M. Löwik, Anika M. Jonker |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Ethylene Polymers and Plastics biology Chemistry technology industry and agriculture Bioengineering Polymer Matrix (biology) biology.organism_classification Biomaterials HeLa chemistry.chemical_compound Cell culture Polymer chemistry Self-healing hydrogels PEG ratio Materials Chemistry Biophysics Cell adhesion Biotechnology |
| Zdroj: | Macromolecular Bioscience. 15:1338-1347 |
| ISSN: | 1616-5187 |
| DOI: | 10.1002/mabi.201500110 |
| Popis: | Poly(ethylene)glycol (PEG)-based hydrogels are often used as matrix material for cell culturing. An efficient method to prepare soft PEG gels is by cross-linking via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). Here, the effect of polymer density and RGDS-content on hydrogel formation and cell adhesion was studied, by varying the total polymer content (10, 20 and 30 mg · mL(-1) ) and the amount of RGDS moieties (0-100%) independently of each other. Rheology studies confirmed the soft nature of the hydrogels (G' = 25-2 298 Pa). HOS cells are able to adhere well to all RGDS-containing gels. Interestingly, both HeLa cells and NIH 3T3 fibroblasts showed substantial adherence to 10 and 20 mg · mL(-1) gels, but with increased hydrogel stiffness (30 mg · mL(-1) ), their cellular adhesion decreased significantly. |
| Databáze: | OpenAIRE |
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