Selectivity-enhancement in enantioselective hydrolysis of sec-alkyl sulfates by an alkylsulfatase from Rhodococcus ruber DSM 44541
| Autor: | Ulrike T. Strauss, Kurt Faber, Mateja Pogorevc, Thomas Riermeier |
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| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Immobilized enzyme Chemistry Metal ions in aqueous solution Organic Chemistry Polyethylene glycol Catalysis Kinetic resolution Inorganic Chemistry Hydrolysis chemistry.chemical_compound Organic chemistry lipids (amino acids peptides and proteins) Physical and Theoretical Chemistry Sulfate Selectivity Alkyl |
| Zdroj: | Tetrahedron: Asymmetry. 13:1443-1447 |
| ISSN: | 0957-4166 |
| DOI: | 10.1016/s0957-4166(02)00363-4 |
| Popis: | The Enantioselectivity of the biohydrolysis of sec -alkyl sulfate esters using a bacterial alkylsulfatase from Rhodococcus ruber DSM 44541 was dramatically enhanced in presence of additives (‘enhancers’) such as carbohydrates, polyethylene glycol, detergents, metal ions and through enzyme immobilization. In presence of iron, the E value for the kinetic resolution of (±)-3- and (±)-4-octyl sulfate was improved from E =3.9 to ≥200 and E =1.1 to 10, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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