Growth ofGeobacter sulfurreducenson Poorly Crystalline Fe(III) Oxyhydroxide Coatings
| Autor: | Paul L. Wincott, Francis R. Livens, David J. Vaughan, Jonathan R. Lloyd, Michael J. Wilkins |
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| Rok vydání: | 2007 |
| Předmět: |
biology
Inorganic chemistry Biofilm chemistry.chemical_element biology.organism_classification Microbiology Chromium Electron transfer Ferrihydrite chemistry X-ray photoelectron spectroscopy Phase (matter) Earth and Planetary Sciences (miscellaneous) Environmental Chemistry Environmental scanning electron microscope Geobacter sulfurreducens General Environmental Science |
| Zdroj: | Geomicrobiology Journal. 24:199-204 |
| ISSN: | 1521-0529 0149-0451 |
| Popis: | Few studies have examined the molecular to micron-scale interactions between dissimilatory Fe(III)-reducing bacteria and poorly crystalline Fe(III) phases which are frequently the most bioavailable Fe(III) sources in the subsurface. Here we describe methods for analysing these interactions using a range of chemical and spectroscopic techniques. Glass slides were coated with a synthetic poorly crystalline Fe(III) phase and then incubated in the presence of the Fe(III)-reducing bacterium Geobacter sulfurreducens and a suitable growth medium. Growth on the Fe(III)-coating was observed via cell staining and environmental scanning electron microscopy while microbial Fe(III) reduction was quantified using a colorimetric assay. However, following microbial reduction, Fe(II) could not be detected on the slide surface using X-ray photoelectron spectroscopy. Fe(II)-coated control slides showed that the mineral surface was not re-oxidised during handling or analysis. Further experiments intended to demonstrate remov... |
| Databáze: | OpenAIRE |
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