Immunomodulator ‘mushroom beta glucan’ induces Wnt/β catenin signalling and improves wound recovery in tilapia and rat skin: a histopathological study
| Autor: | Shiu-Nan Chen, Lynette Chen, Chien-Mei Hsiao, Shih-Ling Huang, Fan-Hua Nan, Yu-Sheng Wu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty biology Cell growth Growth factor medicine.medical_treatment Liver cell Wnt signaling pathway Dermatology biology.organism_classification Beta-glucan Molecular biology In vitro 03 medical and health sciences Nile tilapia chemistry.chemical_compound 030104 developmental biology chemistry medicine Surgery Bromodeoxyuridine |
| Zdroj: | International Wound Journal. 13:1116-1128 |
| ISSN: | 1742-4801 |
| DOI: | 10.1111/iwj.12427 |
| Popis: | The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia (Oreochromis niloticus) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assays and bromodeoxyuridine (BrdU) cell proliferation assay detection method. Results showed that MBG treatment was remarkable in enhancing cell proliferation of hepatocytes and in maintaining the cellular viability. Immunohistochemical staining to analyse Wnt/β-catenin signalling also showed that MBG has the effect of promoting cell proliferation of liver tissues after PH surgery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text