Bioconversion pathways and metabolic profile of daidzin by human intestinal bacteria using UPLC–Q-TOF/MS
| Autor: | Min Zhao, Ling Wang, Jin-hua Tao, Shu Jiang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Human feces
0303 health sciences Chromatography biology 030309 nutrition & dietetics Bioconversion Daidzein 04 agricultural and veterinary sciences General Chemistry biology.organism_classification 040401 food science Biochemistry Industrial and Manufacturing Engineering Hydroxylation 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology chemistry Biotransformation Escherichia Daidzin Bacteria Food Science Biotechnology |
| Zdroj: | European Food Research and Technology. 247:1655-1664 |
| ISSN: | 1438-2385 1438-2377 |
| Popis: | Gut bacteria play a crucial role in the biotransformation of dietary isoflavonoids, which may affect the pharmacological effects of these bioactive compounds in the host. In this study, 67 Escherichia, 16 Enterococcus and 16 Bacillus strains were newly isolated from human feces and their conversion capability of daidzin was first investigated by a rapid and sensitive ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC–Q-TOF/MS) technique with Metabolynx™ software combined with mass defect filtering (MDF) method. Compared with blank samples, daidzein and three new metabolites: methoxylated daidzein (M1), acetylated daidzin (M2), hydroxylated daidzein (M3) were detected and tentatively identified based on the characteristics of their protonated ions. These metabolites indicated that daidzin was first deglycosylated to large amounts of daidzein by almost all the isolated bacteria and subsequently to minor amounts of M1 and M3 through methoxylation and hydroxylation by few bacteria, respectively. Additionally, daidzin could also be acetylated to minor but detectable amounts of M2 by the minority of intestinal bacteria. In the three genera, Escherichia strains were the dominant intestinal bacteria for daidzin conversion. The conversion capability of Escherichia sp. 88 was more powerful than the others. These present data will be helpful for the further pharmacokinetic investigation of daidzin and to unravel how it works in vivo. |
| Databáze: | OpenAIRE |
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