Immunological detection in indoor environments of house dust mite allergens using avian antibodies - IgY: An innovative tool
| Autor: | Angela Espejo, Stephanie Saavedra, Eduardo Egea, Gloria Garavito, Ana Sofía Moreno, Luis A. Barrera, Dary Mendoza |
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| Rok vydání: | 2021 |
| Předmět: |
House dust mite
biology General Mathematics General Physics and Astronomy General Chemistry biology.organism_classification General Biochemistry Genetics and Molecular Biology respiratory tract diseases Microbiology General Energy History and Philosophy of Science immune system diseases biology.protein General Earth and Planetary Sciences Antibody General Agricultural and Biological Sciences |
| Zdroj: | Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. |
| ISSN: | 2382-4980 0370-3908 |
| Popis: | IgY technology has emerged as an alternative for antibody production with immunodiagnostic purposes. Recently, we produced avian antibodies (IgY) against group-1 dust mite allergens of Dermatophagoides sp (Dsp) and Blomia tropicalis (Bt). These allergens are important sensitizing agents that contribute to the pathophysiology of allergy rhinitis and asthma in patients living in tropical areas. The purpose was to explore the utility of IgY for detecting dust mite allergens in indoor environments. We collected 100 samples of dust from mattresses of asthmatic individuals living in two cities on the Colombian Caribbean coast. We used an in-home indirect enzyme-linked immunosorbent assay (ELISA) to detect the mite allergens using two IgYs (IgY-PO2 and IgY-PO4). We determined the IgY detection limit using whole body extracts of Dsp and Bt; likewise, IgY specificity was probed against one crude extract of cockroach, as well as the precision and accuracy of measurements. Both antibodies recognized allergens in the mite extracts, even at the lowest tested concentration (0.05 µg/mL); none reacted with the cockroach extract demonstrating their specificity for mite allergens. On the other hand, IgY-PO4 showed the best precision (intra-assay: CV < 6.99%; inter-assay: CV ≤ 8.71%) and an accuracy: 80.62 – 100.39%. Allergen levels detected were consistent with the mite density present in the dust samples. We confirmed the usefulness of IgY-PO2 and IgYPO4 for detecting dust mite pollution in indoor environments. |
| Databáze: | OpenAIRE |
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