Liposome-Encapsulated Hemoglobin Ameliorates Tumor Hypoxia and Enhances Radiation Therapy to Suppress Tumor Growth in Mice
| Autor: | Chieko Murayama, Sotaro Sadahiro, Nobusuke Kato, Munetaka Haida, Kenji Ishikawa, Akira T. Kawaguchi, Akemi Kamijo, Yukio Ohizumi |
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| Rok vydání: | 2012 |
| Předmět: |
Liposome
medicine.medical_specialty Tumor hypoxia business.industry medicine.medical_treatment Biomedical Engineering Medicine (miscellaneous) Bioengineering General Medicine Tumor Oxygenation Biomaterials Radiation therapy Endocrinology Internal medicine Respiration Immunohistochemistry Medicine Tumor growth Hemoglobin business |
| Zdroj: | Artificial Organs. 36:170-177 |
| ISSN: | 0160-564X |
| Popis: | We hypothesize that liposome-encapsulated hemoglobin with high O₂ affinity (P₅₀0₂ = 12 mm Hg, h-LEH) may increase O₂ delivery to hypoxic tumors and enhance radiation therapy synergistically to suppress tumor growth. First, h-LEH (5, 10, and 20 mL/kg) was intravenously infused 30 min before radiation (20 Gy) of SCCVII tumor grown in C3H/HeN mice. Second, 10 mL/kg of h-LEH was administered 30, 60, 90, and 120 min prior to radiation to determine optimal timing. Tumor size was monitored thereafter to titrate tumor growth suppression. Third, additional mice with SCCVII tumor were infused with h-LEH or empty liposome (EL), and tumors were excised at various time points for immunohistochemical examination of h-LEH and hypoxia-inducible factor-1α (HIF-1α). h-LEH was most effective at 10 mL/kg in comparison to 5 or 20 mL/kg of h-LEH or EL. Tumor growth was most suppressed when the interval between h-LEH infusion and radiation was shortest, 30 min. As a result, 10 mL/kg of h-LEH infusion 30 min prior to radiation prolonged 5-fold tumor-growth time from 20.0 days (radiation and EL) to 26.5 days, P |
| Databáze: | OpenAIRE |
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