Phospholipase C zeta 1 mRNA as a marker of oocyte-activation and fertilization potential of water buffalo (Bubalus bubalis) semen
| Autor: | R. D. Tadeo, E. C. Atabay, Emma V. Venturina, Rafael A. Fissore, Claro N. Mingala, Z. P. Fajardo, E.P. Atabay |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
endocrine system Messenger RNA General Veterinary biology urogenital system 0402 animal and dairy science Semen Oocyte activation 04 agricultural and veterinary sciences urologic and male genital diseases biology.organism_classification 040201 dairy & animal science Cryopreservation Andrology 03 medical and health sciences Semen quality fluids and secretions 030104 developmental biology Real-time polymerase chain reaction Human fertilization Animal Science and Zoology Bubalus |
| Zdroj: | Livestock Science. 225:103-108 |
| ISSN: | 1871-1413 |
| DOI: | 10.1016/j.livsci.2019.04.011 |
| Popis: | The present study aimed to detect and quantify the expression of buffalo PLCZ1 mRNA (buPLCZ1 mRNA) in buffalo semen and to determine its oocyte-activation and fertilizing ability through IVF. Buffalo semen samples were collected and subjected to standard subjective physical and microscopic evaluation of semen characteristics. Small amount of fresh semen sample was taken for molecular experiment. The rest of the samples were processed into frozen semen. Both fresh and frozen semen were subjected to Reverse Transcriptase quantitative PCR (RT-qPCR) technique to determine and quantify buPLCZ1 mRNA expression. Subsequently, 40 bulls with known buPLCZ1 mRNA content were evaluated for its oocyte-activation activity through IVF using frozen semen. Correlation analysis was done to determine the relationship between the expression of buPLCZ1 mRNA and percentage of IVF, and other semen variables. PLCZ1 mRNA expression in buffalo semen was observed variable among donor bulls, but was not significantly different (P > 0.05) between fresh and frozen semen. The result suggests that the cryopreservation procedure does not affect the expression of buPLCZ1 mRNA in semen. Statistical analysis showed that the expression of buPLCZ1 mRNA in fresh semen was strongly correlated with that of frozen semen (r = 0.992; P 0.05) nor with Post-Thaw Motility (r = −0.0313; P > 0.05). A strong relationship between buPLCZ1 mRNA concentration and IVF rate indicates that buPLCZ1 mRNA concentration can be used to assess oocyte-activation and fertilizing potential of the buffalo semen. The present study essentially demonstrated buPLCZ1 mRNA as biological marker for male fertility and that molecular technique serves as an objective approach of semen quality evaluation to enhance bull selection for genetic improvement in water buffaloes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect