Clioquinol and 8-hydroxyquinoline-5-sulfonamide derivatives damage the cell wall ofPythium insidiosum

Autor:	Bruna Pippi, Régis A Zanette, Angélica R Joaquim, Maria E Krummenauer, Simone Merkel, Paula Reginatto, Marilene H Vainstein, Saulo F Andrade, Alexandre M Fuentefria, Juliana S M Tondolo, Érico S Loreto, Janio M Santurio
Rok vydání:	2022
Předmět:	General Medicine Applied Microbiology and Biotechnology Biotechnology
Zdroj:	Journal of Applied Microbiology. 134
ISSN:	1365-2672
DOI:	10.1093/jambio/lxac038
Popis:	AimsTo evaluate the antimicrobial activity and to determine the pharmacodynamic characteristics of three 8-hydroxyquinoline derivatives (8-HQs) against Pythium insidiosum, the causative agent of pythiosis.Methods and ResultsAntimicrobial activity was tested by broth microdilution and MTT assays. The antimicrobial mode of action was investigated using sorbitol protection assay, ergosterol binding assay, and scanning electron microscopy. Clioquinol, PH151, and PH153 were active against all isolates, with MIC values ranging from 0.25 to 2 µg ml-1. They also showed a time- and dose-dependent antimicrobial effect, damaging the P. insidiosum cell wall.ConclusionsTogether, these results reinforce the potential of 8-HQs for developing new drugs to treat pythiosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c1733abbb28e0572391f5d39d2ab5222 https://doi.org/10.1093/jambio/lxac038 Zobrazit plný text záznamu Plný text