| Autor: |
B. J. Bolton, Glyn N. Stacey, Alan Doyle |
| Rok vydání: |
1992 |
| Předmět: |
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| Zdroj: |
Animal Cell Technology: Basic & Applied Aspects ISBN: 9780792318828 |
| DOI: |
10.1007/978-94-011-2844-5_5 |
| Popis: |
DNA fingerprinting is a new technique which provides a highly useful supplement to the standard authentication techniques of isoenzyme analysis and cytogenetics. While fingerprinting does not identify the species of origin it does give specific identification of a cell line. As an alternative, single locus probes can be useful in distinguishing very closely related cell lines. However, they lack the capacity of multilocus DNA fingerprinting whereby a single test can both identify the cell line with good specificty and exclude cross-contamination by cell lines from an extremely wide range of species. We demonstrate the stability of fingerprints of cell lines during repeated sub culture, mycoplasma infection (with two species) and high level antibiotic treatment. This data contributes to the validation of DNA fingerprinting in cell technology and promotes its use for routine quality control of cell stocks of diverse species of origin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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