The Use of Glufosinate as a Selective Marker for the Transformation of Cucumber (Cucumis sativus L.)

Autor:	Liu Jang-Ryol, Cho Mi-Ae, Song Yun-Mi, Min Sung-Ran, Choi Pil-Son, Ko Suck-Min, Park Yun-Ok
Rok vydání:	2005
Předmět:	biology Agrobacterium fungi food and beverages biology.organism_classification Molecular biology chemistry.chemical_compound Transformation (genetics) Glufosinate chemistry Botany Gene Cucumis Selectable marker Explant culture Southern blot
Zdroj:	Journal of Plant Biotechnology. 32:161-165
ISSN:	1598-6365
DOI:	10.5010/jpb.2005.32.3.161
Popis:	Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.
Databáze:	OpenAIRE
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