Single-seed descent, single-pod, and bulk sampling methods for soybean

Autor: Mizuki Funada, J. J. Hammond, Khwaja Hossain, Theodore C. Helms, Curt Doetkott
Rok vydání: 2012
Předmět:
Zdroj: Euphytica. 192:217-226
ISSN: 1573-5060
0014-2336
DOI: 10.1007/s10681-012-0837-3
Popis: The single seed descent (SSD) method of inbreeding minimizes the amount of genetic sampling. The single-pod descent (SPD) and bulk methods (BM) produce redundant inbred lines that are descended from either the same F2 or F3 plant. However, for soybean [Glycine max (L.) Merr.], the SSD method requires more time to process the seed than the SPD or BM. Our experiment is the first to compare the SSD, SPD and BM by sampling the same population in the field and then evaluating the methods using molecular markers. Our objective was to determine the relative efficiency of the SSD, SPD and BM procedures. We defined unique lines as those lines that were not paired with any other line at a coefficient of similarity (Sxy) level ≥ 0.875, which was an alike-in-state criterion. The efficiency was defined as the number of unique lines developed by each procedure. We genotyped 100 F4:5 lines from each of the three genetic sampling methods, using 21 polymorphic simple sequence repeat markers. The number of unique lines was the same for all three sampling methods at the 0.05 level of Type I error. Based on our criterion, the three sampling methods are equally efficient. Our conclusions were the opposite of all other previously published reports. Each breeder will have to determine the best method for generation advancement, based on the amount of resources required to harvest and process the seed.
Databáze: OpenAIRE
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