| Autor: |
Qiu Ping Guo, Xiang Xian Meng, Weihong Tan, Xiaohai Yang, Kemin Wang |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Chinese Chemical Letters. 20:990-994 |
| ISSN: |
1001-8417 |
| DOI: |
10.1016/j.cclet.2009.03.028 |
| Popis: |
Detection of deoxyribozyme (DNAzyme) cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8–17deoxyribozyme (8–17DNAzyme) against hepatitis C virus RNA (HCV-RNA) was designed and the cleavage process was studied in real time. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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