Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice

Autor: Yin Kaisheng, Zhou Lin-fu, Zhou Zhi-min
Rok vydání: 2003
Předmět:
Zdroj: Chinese Journal of Integrative Medicine. 9:281-284
ISSN: 1993-0402
1672-0415
DOI: 10.1007/bf02838615
Popis: Objective: To investigate the distribution and recruitment of pulmonary dendritic cells (DCs) and the influence of low dosage arsenic trioxide (As2O3) on them in the airway of asthmatic mice.Methods: Thirty BALB/c mice were randomly divided into 3 groups: the control group, the asthmatic group and the As2 O3 treated group. The mice asthmatic model was induced via sensitizing with peritoneal injection of ovalbumin (OVA) for two times and then provocated with aerosol inhalation of OVA for a week. The treated group was peritoneally injected with 0. 2 ml solution of As2O3 (4mg/kg) 0. 5h after each provocation. The immunohistochemistry and computerised image analysis were applied to detect quantitatively the DCs in the lung and airway of mice.Results: All intraepithelial nonlymphoid dendritic cells-145 (NLDC-145) throughout the respiratory tree in the mice of the control group formed a network with the density of DCs varying from (575±54) cells/mm2 epithelial surface in the large airway, to (68±12) cells/ mm2 epithelial surface in the small airway. The distribution of airway NLDC-145+ in the asthmatic group was similar to that in the control group, but its density was significantly upregulated (P < 0.01). The distribution of airway NLDC-145 in the treated group was similar to that in the asthmatic group, only its density was significantly downregulated (P < 0.01).Conclusion: There is an integral network of NLDC-145+ throughout the respiratory tree. To downregulate the density but not change the distribution of pulmonary DCs could be an important therapeutic mechanism of low dosage As2 O3 in treating asthma.
Databáze: OpenAIRE
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