585 PB 100 IMPROVED REGENERATION FREQUENCY OF KENTUCKY BLUEGRASS FROM CALLUS CULTURES
| Autor: | Margaret S. Dibble, Jeffrey D. Griffin |
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| Rok vydání: | 1994 |
| Předmět: | |
| Zdroj: | HortScience. 29:515f-515 |
| ISSN: | 2327-9834 0018-5345 |
| DOI: | 10.21273/hortsci.29.5.515f |
| Popis: | Genetic transformation of Kentucky bluegrass (Poa pratensis L.) requires methods for high-frequency regeneration of plantlets from cultured cells. Regeneration of this important turf species has been reported, albeit at a low frequency from seed-derived callus. We tested the potential of 3 synthetic auxins, used in the callus initiation and growth medium, for their ability to promote regeneration in 3 bluegrass varieties. 10 μM 2,4-D promoted regeneration from 0 to 5% of calli, 30 μM and 60 μM picloram promoted regeneration from 0 to 8% of calli, and 10 to 60 μM dicamba, in combination with BA, promoted regeneration from 1% to 8% of calli. In a subsequent experiment, both 60μM and 90 μM dicamba, with 20 μM BA, promoted regeneration from 45% of calli averaged across varieties. These media were tested for the promotion of regeneration in 12 diverse bluegrass varieties. Although up to 45% of the calli from some varieties regenerated plantlets, the response of other varieties was markedly lower, indicating a genetic component in the response to these media. |
| Databáze: | OpenAIRE |
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