Refining the description of protein chromatography

Autor:	Q.S. Yuan, Florian Lode, T.W. Perkins, Edwin N. Lightfoot, Jon Coffman, Thatcher W. Root
Rok vydání:	1997
Předmět:	Packed bed Chromatography Chemistry Organic Chemistry General Medicine Biochemistry High-performance liquid chromatography Column (database) Analytical Chemistry Sphere packing Skewness Protein chromatography Scaling Refining (metallurgy)
Zdroj:	Journal of Chromatography A. 760:139-149
ISSN:	0021-9673
Popis:	Review of design procedures used for protein chromatography shows the need for considerable refinement to meet the demands of high flow-rates and complex processes characteristic of modern commercial operation. Suggestions are made for dealing with the skewness of effluent curves from differential chromatography in interpreting such data and also for scaling up laboratory processes. Proton-specific nuclear magnetic resonance images of small commercial columns show significant non-uniformity of packing density, and suggestions are made for a study of the effects of column shape, size and packing procedures on column efficiency.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c13896ffcba7cb39074a6cd8a8d21159 https://doi.org/10.1016/s0021-9673(96)00843-6 Zobrazit plný text záznamu Full Text from ScienceDirect