Enhancement of glucose metabolism in a pyruvic acid-hyperproducing Escherichia coli mutant defective in F1-ATPase activity

Autor:	Masaru Henmi, Fusao Tomita, Yuji Takezawa, Yoshihiro Fukumori, Naohisa Takaoka, Atsushi Yokota, Chiho Hayashi
Rok vydání:	1997
Předmět:	Strain (chemistry) Glucose uptake PEP group translocation Metabolism Biology medicine.disease_cause Applied Microbiology and Biotechnology chemistry.chemical_compound Biochemistry chemistry medicine Glycolysis Pyruvic acid Escherichia coli Pyruvate kinase Biotechnology
Zdroj:	Journal of Fermentation and Bioengineering. 83:132-138
ISSN:	0922-338X
DOI:	10.1016/s0922-338x(97)83571-4
Popis:	An F1-ATPase-defective mutant of Escherichia coli K-12, strain TBLA-1, showing an enhanced pyruvic acid productivity compared with its parental strain W1485lip2, was characterized physiologically to elucidate the mechanisms of the enhancement in productivity. Cells of strain TBLA-1 producing pyruvic acid showed a higher rate of the oxygen consumption and a higher b-type cytochromes content than cells of strain W1485lip2. The activities of the phosphotransferase system for glucose uptake measured in terms of the level of phosphoenolpyruvate-dependent phosphorylation of methyl α- d -glucopyranoside and 2-deoxy- d -glucose using toluenized cells were higher in strain TBLA-1 than in strain W1485lip2. Among the activities of all the glycolytic enzymes, those of phosphoglycerate kinase and pyruvate kinase-I were found to be higher in strain TBLA-1 than in strain W1485lip2 during the logarithmic growth phase. These characteristics of strain TBLA-1 are discussed in relation to its enhanced pyruvic acid productivity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c137ef00bdbe19dd9eca28a8eeac3ba6 https://doi.org/10.1016/s0922-338x(97)83571-4 Zobrazit plný text záznamu Full Text from ScienceDirect