Novel paper‐based colorimetric immunoassay (PCI) for sensitive and specific detection of salbutamol residues in flesh of swine and urine using Ag 3 PO 4 /Ag nanocomposite as label

Autor: Runglawan Chawengkirttikul, Rattapol Meelapsom, Wipark Anutrasakda, Purim Jarujamrus, Atitaya Siripinyanond, Nutthaporn Malahom, Maliwan Amatatongchai
Rok vydání: 2019
Předmět:
Zdroj: Journal of Food Science. 85:209-219
ISSN: 1750-3841
0022-1147
DOI: 10.1111/1750-3841.14974
Popis: Salbutamol (SAL) can cause potential hazards to human health and its use as a growth promoter in meat-producing animals is illegal. This work reports a novel approach for competitive paper-based colorimetric immunoassay (PCI) using the Ag3 PO4 /Ag nanocomposite as label for sensitive and specific determination of SAL in flesh of swine and urine. The Ag3 PO4 /Ag nanocomposite was synthesized by a one-step chemical bath method, which could instantly oxidize a chromogenic substrate for the color development under acidic conditions without the participation of H2 O2 . This approach provides high affinity between the Ag3 PO4 /Ag nanocomposite and the substrate (with the Michaelis-Menten constant of 0.44 mM). In addition, the fabrication process of the PCI was simple and cost-effective. Particularly, the novel PCI also exhibits simplicity and cost-effectiveness of the fabrication process through a simple wax screen-printing, which requires inexpensive equipment and material including a screen, wax, a squeegee, and a hair dryer. Under optimal conditions, the competitive PCI exhibited a linearity range of 0.025 to 1.00 µg/L. The developed approach offers advantages over the conventional ELISA for the purpose of routine use because it requires a shorter incubation time (
Databáze: OpenAIRE
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