A novel pyrazoline-based fluorescent probe for Cu2+ in aqueous solution and imaging in live cell
| Autor: | Ying-Peng Zhang, Yun-Shang Yang, Qing-Hua Xue, Huichen Guo, Ji-Jun Xue, Yu-chen Zhao |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Aqueous solution Metal ions in aqueous solution Analytical chemistry Pyrazoline 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Ion Inorganic Chemistry chemistry.chemical_compound Resist chemistry Materials Chemistry Physical and Theoretical Chemistry 0210 nano-technology Selectivity |
| Zdroj: | Inorganic Chemistry Communications. 129:108612 |
| ISSN: | 1387-7003 |
| Popis: | In this study, a new pyrazoline derivate (1-acetyl-3- (2-hydroxy-1-naphthyl)-5-(2-phenyl-2H-chromene)-2-pyrazoline, N) is described as fluorescent probe for fluorometric detection of Cu2+. This fluorescence of probe has a high selectivity only toward Cu2+ ion in the other metal ions. It is remarkably observed that the fluorescence intensity of N decreased in the presence of Cu2+ ions with a strong capability to resist perturbations. The Limit of detection (LOD) value is calculated as 8.85 × 10−7 M for Cu2+ while the complexation constant is calculated as 3.14 × 105 M−2 via fluorescence measurements. The interaction between of N and Cu2+ is reversible after adding PPi. The optimized calculation of the probe for Cu2+ is confirmed by TD-DFT calculations. N-Cu2+ is successfully applied in the intracellular imaging in living PK-21cells that will have a great chance for wide application. |
| Databáze: | OpenAIRE |
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