[16] Identification and characterization of central nervous system peptidase activities

Autor:	John R. McDermott, Alison M. Gibson
Rok vydání:	1995
Předmět:	Serine medicine.anatomical_structure Biochemistry Metallopeptidase Stereochemistry Cleave Central nervous system biology.protein medicine Human brain Biology Amyloid precursor protein secretase Endopeptidase
DOI:	10.1016/s1043-9471(06)80127-8
Popis:	Summary Synthetic peptides from β-amyloid precursor protein were used as substrates to identify and characterize four peptidase activities in human brain, as follows: o 1. A novel Mn 2+ -stimulated metallopeptidase with the specificity to cleave at the Lys-Leu secretase site in APP (1). 2. Peptidase with the specificity to generate the N-terminus of β-AP from APP by cleaving a Met-Asp (or Leu-Asp) bond: characterization showed this activity to be endopeptidase 24.15 (2). 3. Two membrane peptidases with the specificity to cleave Phe 19 -Phe 20 and Ala 21 -Glu 22 in β-AP; the former metal dependent and the latter serine dependent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c11e9083ca1d2da48424d546f682cef9 https://doi.org/10.1016/s1043-9471(06)80127-8 Zobrazit plný text záznamu