Investigation of Approaches for the Fabrication of Protein Patterns by Scanning Probe Lithography
| Autor: | Jeremy R. Kenseth, Marc D. Porter, and Vivian W. Jones, Jennifer A. Harnisch |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Langmuir. 17:4105-4112 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | This paper investigates three different approaches to patterning proteins within ultrathin resist layers formed from self-assembled monolayers using scanning probe lithography (SPL) at the submicrometer length scale. The first approach uses a “nanografting” method to pattern a reactive carboxylic acid terminated thiol into a resist composed of a methyl-terminated monolayer. Rabbit IgG antigen is bound to the patterned region, and an immunoassay utilizing direct readout of the topographic change resulting from specific binding of anti-rabbit IgG antibody is performed using scanning force microscopy. To address issues related to nonspecific protein adsorption, the other two approaches investigated the patterned removal of glycol-terminated monolayers by mechanically “scraping” patterns at high tip−sample forces by SPL. Protein attachment to the scraped regions was achieved either through the chemisorption of a disulfide coupling agent or by the direct adsorption of Fab‘-SH antibody fragments. Results obtain... |
| Databáze: | OpenAIRE |
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