Isolation and characterization of twenty-one novel microsatellite loci for the black cicada, Cryptotympana atrata (Hemiptera: Cicadidae)

Autor: Honghai Zhang, Haotian Li, Shuyu Xue, Wanchao Zhu, Tianshu Lv, Jun Chen, Huanxin Zhang
Rok vydání: 2016
Předmět:
Zdroj: Applied Entomology and Zoology. 51:511-514
ISSN: 1347-605X
0003-6862
DOI: 10.1007/s13355-016-0424-5
Popis: To study population genetics and facilitate species identification, we developed 21 polymorphic microsatellite loci for Cryptotympana atrata (Fabricius), a widespread insect that makes a loud, high-pitched noise. Polymorphisms detected in 28 individuals ranged from 2 to 18 alleles per locus. The observed and expected heterozygosity ranged from 0.0714 to 0.9286 and from 0.0689 to 0.9082, respectively. The polymorphic information content value ranged from about 0.0670 to 0.9017. One locus showed low polymorphism (PIC 0.5). Three loci showed significant deviations from Hardy–Weinberg equilibrium after Bonferroni correction. These microsatellite markers will be useful tools for the conservation genetic study of this species.
Databáze: OpenAIRE