Ecotoxicological assessment of metalworking fluids using theAllium cepatest procedure
| Autor: | Sefa Pekol |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Mitotic index
Ecology Test procedures Biology medicine.disease_cause biology.organism_classification Toxicology medicine General Earth and Planetary Sciences Bioassay Allium Food science History of use Micronucleus Ecology Evolution Behavior and Systematics Genotoxicity General Environmental Science EC50 |
| Zdroj: | Chemistry and Ecology. 30:66-75 |
| ISSN: | 1029-0370 0275-7540 |
| DOI: | 10.1080/02757540.2013.831079 |
| Popis: | Metalworking fluids (MWFs), which have a long history of use in industry and are in constant and ever-increasing use in parallel with advancements in mechanisation, are emulsions prepared with water. MWFs prepared as a 1/20 (MWF: water) mixture in practice, contain a large amount of water within their structure, and may become an aquatic toxic mixture in an ecosystem. In this study, half maximal effective concentration (EC50) values for MWFs were determined using the Allium cepa test, an accepted ecotoxicological biomonitor, and test solutions were prepared (1/250, 1/500 and 1/1000). Depending on the MWF ratio at each concentration, a decrease in the mitotic index (MI), irregularities in the phase distribution and aberrations in the chromosomes were observed. When the amount of MWF in the water increased, chromosomal abnormalities such as stickiness were observed to occur; whereas abnormalities such as c-mitosis, fragments, bridges, vagrants and micronucleus increased as the amount of the MWF decreased. O... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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