EVALUATION OF THE FLOW CYTOMETRIC PARAMETERS MEASURING CD34+ CELLS IN CORD BLOOD 68

Autor:	C Stavropoulos-Giokas, F Debonera, D. Skoumi, A. Germenis, G Paterakis
Rok vydání:	1997
Předmět:	Pathology medicine.medical_specialty education.field_of_study Absolute number Cd34 cells Population CD34 Biology Molecular biology Fluorescence White Cell Cord blood Pediatrics Perinatology and Child Health medicine Stem cell education
Zdroj:	Pediatric Research. 41:755-755
ISSN:	1530-0447 0031-3998
DOI:	10.1203/00006450-199705000-00087
Popis:	This study was scheduled to evaluate the different flow cytometric parameters used for the measurement of the absolute number of CD34+ cells in cord blood (CB). In samples of 86 CB units, the following parameters were determined by the use of the Coulter-XL flow cytometer: [a] total CD34+ cells,[b] low side scatter, bright fluorescence CD34+ cells (CD34++), [c] CD34+/CD45- cells. The percentage of the above CD34+ cells was determined both among the total cell population and the CD45+ white blood cells. The absolute number of CD34+ cells was determined on the cell concentration in each CB unit, as it was measured by the hematologic analyzer Cell-Dyn 3500 (Abbott), both in WIC (white cell impedence count) and WOCR (white cell optical countlysis resistant) channels. The analysis of the results indicated that:[a] The absolute number of CD34++ cells (83 +/- 56×106) and CD34+/CD45- cells (95 +/- 77×106), which commonly correspond to the stem cells, is significantly (p
Databáze:	OpenAIRE
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