| Autor: |
Rini Savitri Daulay, Rina Amalia C. Saragih, Ridwan Muchtar Daulay, Ratna Akbari Ganie, Gino Tann, Bambang Supriyatno |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Open Access Macedonian Journal of Medical Sciences. 9:1057-1060 |
| ISSN: |
1857-9655 |
| DOI: |
10.3889/oamjms.2021.7441 |
| Popis: |
BACKGROUND: Tuberculosis (TB) remains to be a leading cause of morbidity and mortality worldwide. The immune defense against Mycobacterium tuberculosis (M. tuberculosis) is complicated. Interferon gamma (IFN-g) is the main cytokine involved in the immune response of TB. To date, the role of +874 A/T single nucleotide polymorphism (SNP) and TB disease susceptibility continue to be controversial. OBJECTIVES: The aim of this study was to investigate the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia METHODS: A case control study was conducted in Medan and Batubara, North Sumatera, Indonesia from January to December 2016. A total of 51 children with TB and 51 healthy controls were enrolled in this study. Subjects were 2 months to 14 years old age children diagnosed with TB and written informed consent from the parents or the caregivers to participate. Subjects were withdrawn from the study when immunodeficiency condition was found or suffered from other infection disease. DNA samples were obtained from all of the subjects. +874 A/T SNP was identified by performing the amplification refractory mutational system - polymerase chain reaction (ARMS-PCR) method. IFN-g levels were measured by using human enzyme-linked immunosorbent assay/ELISA. Data analysis was performed using chi square and Mann Whitney test. p value |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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