Detection of Influenza Virus with Specific Subtype by Using Localized Surface Plasmons Excited on a Flat Metal Surface
| Autor: | Kotaro Nagata, Akira Ainai, Hiroshi Kano, Hideki Hasegawa, Jun Ning |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Surface (mathematics)
medicine.drug_class Chemistry viruses Surface plasmon General Engineering Analytical chemistry General Physics and Astronomy Monoclonal antibody Molecular physics Virus Metal Excited state visual_art medicine visual_art.visual_art_medium Refractive index Localized surface plasmon |
| Zdroj: | Japanese Journal of Applied Physics. 52:082402 |
| ISSN: | 1347-4065 0021-4922 |
| DOI: | 10.7567/jjap.52.082402 |
| Popis: | We report on a method to determine subtype of influenza viruses by using surface plasmons localized in microscopic region on a flat metal surface. In this method, refractive index variation arisen from interactions between viruses and their monoclonal antibodies is measured. The developed sensor shows stability of refractive index in the order of 10-4 against sample exchange. In our experiment, A/H1N1 viruses are distinguished from A/H3N2 viruses by using monoclonal antibodies immobilized on the metal surface. Since the measurement probe has the volume of ∼6 al, the method has potential to handle multiple subtypes in the measurement of a sample with ultra small volume. |
| Databáze: | OpenAIRE |
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