Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line

Autor: DU Qingyun, Liang Huali, Ni Zheng, Yun Tao, Liu GuangQing, Hua Jionggang, Chen Jianping, Sheng Zutian, Zhang Yu-ying, Li ShuangMao
Rok vydání: 2006
Předmět:
Zdroj: Chinese Science Bulletin. 51:1698-1702
ISSN: 1861-9541
1001-6538
DOI: 10.1007/s11434-006-2044-x
Popis: Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus (RHDV), the in vitro transcripts are introduced into RK13 cells. 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 104.6/mL. Immune electron microscopic observation of the virus particles revealed that the particles were rotund with a diameter of about 30 nm. Besides, virus titre quantification obtained by qRT-PCR showed a correlation between time from infection and virus titre. All these results showed that we have recovered RHDV from RK13 cells by reverse genetics technology successfully, and this would be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and new type vaccines of RHDV.
Databáze: OpenAIRE
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