Alterations of Antioxidant Status and Mitochondrial Succinate Dehydrogenase Activity in the Liver of Wistar Strain Albino Rats Treated with by Ethanol Extracts ofAnnona senegalensisPers (Annonaceae) Stem Bark
| Autor: | Bathlomew Brai, Abraham Ijaola, Naimat Kolawole, Lateef Adegboyega Sulaimon, Rahmat Adetutu Adisa |
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| Rok vydání: | 2019 |
| Předmět: |
biology
Health Toxicology and Mutagenesis Succinate dehydrogenase 010501 environmental sciences Pharmacology Toxicology medicine.disease_cause Malondialdehyde biology.organism_classification 030226 pharmacology & pharmacy 01 natural sciences Acute toxicity Nephrotoxicity Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Toxicity medicine biology.protein Annona senegalensis Oxidative stress 0105 earth and related environmental sciences |
| Zdroj: | Toxicological Research. 35:13-24 |
| ISSN: | 2234-2753 1976-8257 |
| DOI: | 10.5487/tr.2019.35.1.013 |
| Popis: | Numerous ethnomedicinal uses have been attributed to different parts of Annona senegalensis (ASE), including its uses as food and food additives. The present study investigated toxicological and antioxidant effects of 28 days administration of ethanol extracts of ASE stem bark to Wistar strain albino rats. Acute toxicity test was done to determine lethal dose in Wistar rats while sub- acute toxicity test was conducted on rats divided into four groups (A - control, B - 50 mg/kg, C - 100 mg/kg, D - 150 mg/kg, respectively and treated for 28 days. Oxidative stress markers in liver and kidney as well as hepatic succinate dehydrogenase activity in the mitochondrial and post mitochondrial fractions (PMF) were evaluated. The LD50 value of ASE was > 2,000 mg/kg. White blood cell counts gradually increased, but red blood cell counts and haematocrits level decreased significantly (p < 0.05) by about 50%. Liver enzymes in the serum and mitochondrial succinate dehydrogenase activity increased significantly (p < 0.05). Superoxide dismutase and catalase activities also increased in liver mitochondria and PMF while malondialdehyde (MDA) and reduced glutathione levels increased only in the PMF. Furthermore, only MDA levels increased significantly in the kidney after 28 days extract administration. Histopathological examination showed hepatic necrosis and no obvious signs of nephrotoxicity. Anona senegalensis is relatively safe, but prolonged ingestion could induce oxidative stress and impair ATP synthesis through the modulation of the activity of mitochondrial succinate dehydrogenase. |
| Databáze: | OpenAIRE |
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