Synthesis and study of novel pH-independent fluorescent mitochondrial labels based on Rhodamine B
| Autor: | Soňa Krupkova, Kamil Motyka, Ludmila Hradilová, Maitia Labora, Martin Mistrik, Jiři Bartek, Jan Hlaváč, Lucie Brulíková |
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| Rok vydání: | 2016 |
| Předmět: |
Pyrimidine
010405 organic chemistry General Chemical Engineering General Chemistry Mitochondrion 010402 general chemistry 01 natural sciences Fluorescence 0104 chemical sciences Cell membrane Rhodamine chemistry.chemical_compound medicine.anatomical_structure Membrane Biochemistry chemistry medicine Rhodamine B Molecular probe |
| Zdroj: | RSC Advances. 6:23242-23251 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c5ra20183g |
| Popis: | Several Rhodamine B derivatives based on a tri-substituted pyrimidine core were prepared using solid-phase chemistry with a combinatorial approach. These compounds were screened for their basic fluorescence properties and their ability to penetrate through eukaryotic cell membranes. Most can penetrate through the cell membrane and specifically accumulate in mitochondria. Notably, some of our new rhodamine derivatives showed partially sustained mitochondrial localization and exhibited fluorescence at high pH, making them promising candidates for molecular probes to elucidate mitochondrial biology and pathology. |
| Databáze: | OpenAIRE |
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