Influence of mobile phase composition on the adsorption of hen egg-white proteins to anion-exchange cellulose

Autor:	D. Carcary, P. R. Levison, S. E. Badger, B. N. Brook, D. W. Toome
Rok vydání:	1989
Předmět:	Chromatography Ion exchange Hydrophilic interaction chromatography Organic Chemistry Clinical Biochemistry Fast protein liquid chromatography Raw material Biochemistry Analytical Chemistry chemistry.chemical_compound Adsorption Column chromatography chemistry Ionic strength Cellulose
Zdroj:	Chromatographia. 28:170-178
ISSN:	1612-1112 0009-5893
Popis:	Factors influencing the capacity of Whatman QA52 anion-exchange cellulose for hen egg-white proteins have been investigated. While simply increasing the concentration of protein in the feedstock increases the loading there is a concomitant reduction in binding efficiency in a single pass through the column. Substitution of buffer by water in the mobile phase during sample preparation, thereby reducing the ionic strength of the feedstock, had the effect of significantly increasing protein capacity whilst improving binding efficiency. During column loading large changes in pH of the mobile phase were observed although this had no apparent effect on the chromatography for this system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c093acb18802c07e8dcc0ce4152d74aa https://doi.org/10.1007/bf02319642 Zobrazit plný text záznamu Full text from SpringerLink