333. Vector Development by Molecular Evolution: A Selection Procedure for Envelope Proteins Pseudotyping Lentiviral Particles
| Autor: | Joern Stitz, Christian J. Buchholz, Christoph A. Merten, Klaus Cichutek |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pharmacology
chemistry.chemical_classification viruses Heterologous Lipid bilayer fusion Computational biology Biology Virology Amino acid Transduction (genetics) chemistry Cytoplasm Molecular evolution Drug Discovery Gibbon ape leukaemia virus Genetics Pseudotyping Molecular Medicine Molecular Biology |
| Zdroj: | Molecular Therapy. 9:S126 |
| ISSN: | 1525-0016 |
| Popis: | Background: Pseudotyping with heterologous envelope proteins is extensively used to generate lentiviral vectors. However, the molecular determinants in the cytoplasmic tails (CTs) of Env proteins governing particle incorporation and membrane fusion are not well characterised. We established a systematic approach allowing the selection of variants mediating optimised transduction rates of lentiviral vectors from libraries of diversified Env proteins. To set up the system we chose the gibbon ape leukaemia virus (GaLV) Env which is unable to mediate transduction of HIV vector particles. However, this block can be overcome by exchanging three amino acids located in the CT of Env. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|