The phenotypic and genetic characterization of some virulence factors in MRSA isolated from burn patients

Autor:	Abbas Mayar Hezam
Rok vydání:	2019
Předmět:	Genetics History Virulence biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses Phenotype Computer Science Applications Education
Zdroj:	Journal of Physics: Conference Series. 1294:062061
ISSN:	1742-6596 1742-6588
DOI:	10.1088/1742-6596/1294/6/062061
Popis:	The present study aimed to the phenotypic and genetic detection of some virulence factors in Methicillin resistant Staphylococcus aureus (MRSA) isolated from burn patients. from a total of 38 isolates of Staphylococcus aureus, 15(39.4%) isolates of MRSA were collected from the patients in Diwaniyah Teaching Hospital during the period from June to October, 2018 in AL-Diwaniyah City. Methicillin resistant Staphylococcus aureus (MRSA) was detected by using chrom agar MRSA and polymerase chain reaction (PCR), 15 isolates (39.4%) reported resistance to methicillin antibiotic.In addition, phenotypic detection of the ability of MRSA to produce the ß-lactamase enzymes using rapid iodometric method and its ability to form the biofilm was determined by using the tube method, The results showed 15(100%) MRSA isolates were able to produce ß-lactamase enzymes and 14 (93.3%) MRSA isolates were able to biofilm production. On the other hand, All MRSA isolates were tested by using Polymerase Chain Reaction (PCR) to detection of some virulence factors genes, which include coa gene encoding production of coagulase, icaA gene encoding for biofilm production and blaZ gene encoding for the ß-lactamase enzymes. PCR results showed that MRSA isolates were posses blaZ gene (100%), icaA gene (93.3%) and coa gene (86.6%).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c081ad6fc47526626fce00715a27c653 https://doi.org/10.1088/1742-6596/1294/6/062061 Zobrazit plný text záznamu