Fluorometric Determination of Artemisinin Using the Pyronin B–Microperoxidase-11 System

Autor:	E. S. Vakhraneva, Tatyana N. Shekhovtsova, S. V. Muginova, Dina Myasnikova
Rok vydání:	2019
Předmět:	Quenching (fluorescence) Chromatography biology Chemistry 010401 analytical chemistry Artemisia annua Cationic polymerization 010402 general chemistry biology.organism_classification Mass spectrometry 01 natural sciences Horseradish peroxidase 0104 chemical sciences Analytical Chemistry biology.protein medicine Artemisinin Selectivity Peroxidase medicine.drug
Zdroj:	Journal of Analytical Chemistry. 74:100-107
ISSN:	1608-3199 1061-9348
DOI:	10.1134/s1061934818120079
Popis:	A sensitive, rapid, and simple fluorimetric procedure for the determination of artemisinin in a concentration range of 0.1–7 μM was developed with the use of microperoxidase-11 as a peroxidase biomimetic (RSD = 0.8% at LOQ, n = 5; LOD = 7.1 nM (3s0)). The determination is based on the fluorescence quenching of the cationic xanthene dye pyronin B (Stern–Volmer quenching constant, 0.101 μM–1) in the presence of microperoxidase-11. The procedure was tested in the analysis of a biologically active additive based on an Artemisia annua wormwood extract. The correctness of the results of the fluorimetric determination of artemisinin in a biologically active dietary supplement was confirmed by HPLC–mass spectrometry. The use of oligopeptide microperoxidase-11 instead of heme-containing proteins (hemoglobin, cytochrome c, and horseradish peroxidase) made it possible to shorten the duration of artemisinin determination by a factor of 2 with the retention of sensitivity and selectivity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c06b13151766fd33d1a638c03f8d5beb https://doi.org/10.1134/s1061934818120079 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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