Simplified Technic for Estimation of Serum Lipoprotein Cholesterol

Autor:	R. C. Jung, Ronald L. Searcy, L. M. Bergquist, R.G. Craig, Korotzer Jl
Rok vydání:	1960
Předmět:	Chromatography Age differences Filter paper Cholesterol Biochemistry (medical) Clinical Biochemistry Alpha (ethology) chemistry.chemical_compound chemistry Reagent Phosphomolybdic acid lipids (amino acids peptides and proteins) Colorimetric analysis Lipoprotein cholesterol
Zdroj:	Clinical Chemistry. 6:585-597
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/6.6.585
Popis:	A phosphomolybdic acid reagent was used to identify serum alpha- and beta-lipoproteins separated electrophoretically on filter paper. Cholesterol associated with these areas on unstained strips was eluted directly into a ferrous sulfate-acetic acid mixture for colorimetric analysis. The results obtained with the proposed method compared favorably with those of Nury and Smith (7). The sums of the values obtained for the alpha- and beta-lipoprotein cholesterols agreed well with measurements of serum total cholesterol by another method (12). Sex and age differences in the concentration of alpha- and beta-lipoprotein cholesterol were suggested by results obtained on 200 randomly selected blood donors.
Databáze:	OpenAIRE
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