Effects of seed priming on biochemical parameters and seed germination in cowpea [vigna unguiculata (L.) Walp]
| Autor: | S. Shankar Hebbar, K. Bhanuprakash, T. Senthivel, M.N. Arun |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology food and beverages Soil Science Plant Science Priming (agriculture) biology.organism_classification 01 natural sciences Esterase Vigna 03 medical and health sciences Horticulture 030104 developmental biology Agronomy Dry weight Seedling Germination biology.protein Amylase Agronomy and Crop Science 010606 plant biology & botany Peroxidase |
| Zdroj: | LEGUME RESEARCH - AN INTERNATIONAL JOURNAL. |
| ISSN: | 0976-0571 0250-5371 |
| DOI: | 10.18805/lr.v0i0.7857 |
| Popis: | Cowpea seeds lose their vigour during storage at high temperature and high relative humidity. Present study was conducted to invigorate the performance of high and low vigour seeds (exposed to 45 ± 2o C and 100% RH) by seed priming with different chemicals and concentrations (GA3 (100ppm); CaCl2 (10-3M); Ammonium Molybdate (10-3M); KBr (10-3M); Mg (NO3)2 (10-3M); ZnSO4 (10-3M); hydro primed and dry seed (control) at 15o C for 24 hours. Priming was effective in reducing the time for 50 % germination and mean germination time and increased the germination percentage in low vigour seeds, while energy of germination and final germination percentage in normal seeds displayed a minor increase. Priming of normal / low – vigour seeds improved the vigour of seedling in terms of seedling length and their dry weight and seedling vigour index. Seed priming of fresh and accelerated cowpea seeds increased the electrical conductivity of seed leachate, cell membrane stability, total protein content, a – amylase activity, peroxidase activity and dehydrogenase activity over control dry seeds. Esterase and peroxidase enzymes were completely lost as a result of accelerated ageing, which showed reappearance after priming. Priming showed little improvement in the banding pattern and intensity of enzyme in normal seeds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|