Antioxidant and anxiolytic effect of Bifidobacterium adolescentis and Lactobacillus acidophilus under conditions of normobaric hypoxia with hypercapnia

Autor: S. V. Kravchenko, A A Kravtsov, L. I. Ivashchenko, S V Kozin
Rok vydání: 2021
Předmět:
Zdroj: Problems of Nutrition. 90:63-72
ISSN: 2658-7440
0042-8833
DOI: 10.33029/0042-8833-2021-90-2-63-72
Popis: Research in recent years has shown that there is a close connection between the brain and the intestine through neuronal, endocrine and immune pathways. The introduction of probiotics into the diet of animals and humans helps to reduce the level of anxiety and depression, as well as inflammatory processes during emotional stress. The aim of this work was to study the effect of intragastric administration of Bifidobacterium adolescentis and Lactobacillus acidophilus on oxidative processes in the brain tissues and the level of anxiety in rats under conditions of normoxia and acute hypoxia with hypercapnia. Material and methods. The experiment was performed on 64 male Wistar rats aged 2.5 months (body weight from 240 to 270 g). The animals were divided into 4 groups: group 1 - control; 2 - hypoxia; 3 - hypoxia + probiotics; 4 - probiotics. There were 16 animals in each group; half of them participated in the behavioral test, and the other half in the biochemical studies. Rats of groups 3 and 4 were orally administered lyophilized bacteria Bifidobacterium adolescentis MC-42, Lactobacillus acidophilus A-97, and Lactobacillus acidophilus A-630 for 30 days before hypoxia. The daily dose of probiotics was 1×109 CFU per animal, administered in a volume of 1 ml. Acute hypoxia with hypercapnia was simulated by placing rats in airtight vessels with a capacity of 1 L before the first agonal inhalation. A day later, in the brain tissues oxidative processes were assessed by the chemiluminescence method and by the level of malone dialdehyde (MDA). The activity of catalase in brain tissues was also determined. The level of anxiety of rats was investigated in the «elevated plus maze» test. Results. Compared to other groups, more intensive free radical oxidation took place in the brain tissues of hypoxified animals that did not receive B. adolescentis and L. acidophilus. There was a significant increase in chemiluminescence intensity and MDA level by 38 and 15%, respectively, compared with the control. In the brain tissues of these animals, catalase activity was reduced by 10% (p
Databáze: OpenAIRE
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