Structural Development during Germination of Different Populations of Mitochondria from Pea Cotyledons

Autor:	Mary S. Spencer, S. S. Malhotra
Rok vydání:	1973
Předmět:	Physiology food and beverages Plant Science Sucrose gradient Oxidative phosphorylation Cycloheximide Mitochondrion Biology Membrane synthesis Fully developed chemistry.chemical_compound chemistry Biochemistry Germination Genetics Inner mitochondrial membrane
Zdroj:	Plant Physiology. 52:575-579
ISSN:	1532-2548 0032-0889
Popis:	The crude mitochondrial fraction from pea cotyledons can, from days 1 to 7 of germination, be separated into three fractions by sucrose density gradient centrifugation. When seeds were grown in water (control) or cycloheximide (120 micrograms per milliliter of medium) for 4 days, the originally different populations of mitochondria acquired a uniform density and separated together in band 1 (density, 1.205 grams per milliliter). The oxidative and phosphorylative activities of mitochondria obtained from 4-day-old control and 4-day-old cycloheximide-treated pea seeds were the same. However, mitochondria from pea seeds that were grown in d-threo-chloramphenicol (1.5 milligrams per milliliter of medium) or erythromycin (0.5 milligram per milliliter of medium) for 4 days separate into three bands (fully developed mitochondria in the top band [band 1] and partially developed mitochondria in the lower two bands [bands 2 and 3]). Separation patterns and oxidative and phosphorylative activities were the same for mitochondria separated from 4-day-old cotyledons treated with d-threo-chloramphenicol or erythromycin and from 1-day-old cotyledons grown in water. This indicated that these inhibitors prevented the partially developed mitochondria originally in bands 2 and 3 from developing further. In contrast, cycloheximide did not seem to interfere with the mitochondrial structural development. These results along with those obtained from the experiments on the effects of d-threo-chloramphenicol, erthromycin, and cycloheximide on 14C-leucine incorporation into mitochondrial membrane proteins suggest that the increase in mitochondrial activity during germination may be a result of structural development (membrane synthesis) in pre-existing mitochondria.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c049847c7df3eeb31fdf6b5e59193d64 https://doi.org/10.1104/pp.52.6.575 Zobrazit plný text záznamu