Formate dehydrogenase in Arabidopsis thaliana: overexpression and subcellular localization in leaves

Autor:	Håkon Ramberg, Patricia L. Herman, Renee D. Baack, John C. Osterman, John Markwell
Rok vydání:	2002
Předmět:	biology Nicotiana tabacum fungi food and beverages Plant Science General Medicine biology.organism_classification Formate dehydrogenase Enzyme assay Formate oxidation Chloroplast Biochemistry Arabidopsis Genetics biology.protein Arabidopsis thaliana NAD+ kinase Agronomy and Crop Science
Zdroj:	Plant Science. 163:1137-1145
ISSN:	0168-9452
DOI:	10.1016/s0168-9452(02)00326-6
Popis:	Formate dehydrogenase (FDH; EC 1.2.1.2) is a NAD-dependent enzyme that catalyzes the oxidation of formate to carbon dioxide in the mitochondria of higher plants. Sequence analyses and other preliminary experiments suggested that FDH might also be targeted to the chloroplasts of Arabidopsis thaliana and other plant species. In the present study, transgenic Arabidopsis and tobacco plants that overexpress Arabidopsis FDH were produced. The FDH specific activity in the leaf tissue of the transgenic plants increased an average of 4.5-fold for Arabidopsis and 31.5-fold for tobacco. Immunodetection and enzyme assays of intact chloroplasts fractionated from the leaves of transgenic tobacco plants suggested that Arabidopsis FDH is present in the chloroplast. Immunogold labeling of Arabidopsis and tobacco detected FDH in both the mitochondria and chloroplasts of the leaf cells. Enzyme assays that were performed to confirm the specificity of Arabidopsis FDH for the NAD cofactor suggested that NADPH was an inhibitor of NAD + dependent formate oxidation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c047ea9870883fb4c0e485bf28173e89 https://doi.org/10.1016/s0168-9452(02)00326-6 Zobrazit plný text záznamu Full Text from ScienceDirect