Antiallodynic effect induced by [6]-gingerol in neuropathic rats is mediated by activation of the serotoninergic system and the nitric oxide-cyclic guanosine monophosphate-adenosine triphosphate-sensitive K+ channel pathway
| Autor: | Espinosa de Los Monteros-Zuñiga, Alfonso Mata-Bermudez, Teresa Izquierdo, Arrigo Coen, Beatriz Godínez-Chaparro |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pharmacology Opioidergic medicine.drug_class Antagonist (+)-Naloxone Receptor antagonist Nitric oxide 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Opioid receptor medicine Channel blocker Cyclic guanosine monophosphate 030217 neurology & neurosurgery |
| Zdroj: | Phytotherapy Research. 32:2520-2530 |
| ISSN: | 0951-418X |
| DOI: | 10.1002/ptr.6191 |
| Popis: | The present study evaluated the possible antiallodynic effect induced by [6]-gingerol in rats with L5-L6 spinal nerve ligation (SNL). Moreover, we determined the possible mechanism underlying the antiallodynic effect induced by [6]-gingerol in neuropathic rats. The animals underwent L5-L6 SNL for the purpose of developing tactile allodynia. Tactile allodynia was measured with von Frey filaments. Intrathecal administration of [6]-gingerol reversed SNL-induced tactile allodynia. The [6]-gingerol-induced antiallodynic effect was prevented by the intrathecal administration of methiothepin (30 μg per rat; nonselective 5-hydroxytryptamine [5-HT] antagonist), WAY-100635 (6 μg per rat; selective 5-HT1A receptor antagonist), SB-224289 (5 μg per rat; selective 5-HT1B receptor antagonist), BRL-15572 (4 μg per rat; selective 5-HT1D receptor antagonist), and SB-659551 (6 μg per rat; selective 5-HT5A receptor antagonist), but naloxone (50 μg per rat; nonselective opioid receptor antagonist) did not prevent the [6]-gingerol-induced antiallodynic effect. Moreover, intrathecal administration of Nω-nitro-l-arginine methyl ester (100 μg per rat; nonselective nitric oxide [NO] synthase inhibitor), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (10 μg per rat; inhibitor of guanylate cyclase), and glibenclamide (50 μg per rat; channel blocker of adenosine triphosphate [ATP]-sensitive K+ channels) prevented the [6]-gingerol-induced antiallodynic effect. These data suggest that the antiallodynic effect induced by [6]-gingerol is mediated by the serotoninergic system involving the activation of 5-HT1A/1B/1D/5A receptors, as well as the NO-cyclic guanosine monophosphate-ATP-sensitive K+ channel pathway but not by the opioidergic system. |
| Databáze: | OpenAIRE |
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