Characterization of Hsd Plasmid in Salmonella Typhi

Autor:	Naoko Ishiwata, Akio Tanimura, Katsutoshi Mise, Michiko Miyahara
Rok vydání:	1995
Předmět:	viruses General Medicine Biology Salmonella typhi biology.organism_classification Virology Enterobacteriaceae Microbiology chemistry.chemical_compound Plasmid Phage types Restriction map chemistry Lysogenic cycle DNA Phage typing
Zdroj:	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi). 36:404-408_1
ISSN:	1882-1006 0015-6426
DOI:	10.3358/shokueishi.36.404
Popis:	An Hsd (host specificity for DNA) plasmid designated pSTd4 was found in Salmonella Typhi D4, one of the standard phage typing strains. The plasmid was introduced into E. coli K-12, and the restriction map of this plasmid was constructed. The efficiency of plating of λ·0 phage was drastically reduced with pSTd4 as well as other small Hsd plasmids of Enterobacteriaceae origin. The results clearly indicate that pSTd4 is a type-determining plasmid in S. Typhi D4. We propose that rare phage types such as D4 carrying typedetermining plasmids or lysogenic phages should be eliminated from the collection of standard phage typing strains of S. Typhi.
Databáze:	OpenAIRE
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