Surface modification of protein enhances encapsulation in chitosan nanoparticles
| Autor: | Rafael Vazquez-Duhalt, Mariana Andrade, Rina D. Koyani, Paul Gaytán, Katrin Quester, Alejandro Huerta-Saquero |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials Science (miscellaneous)
Nanochemistry Nanoparticle macromolecular substances 02 engineering and technology Nanoreactor 010402 general chemistry 01 natural sciences Chitosan chemistry.chemical_compound Electrical and Electronic Engineering Physical and Theoretical Chemistry technology industry and agriculture Chemical modification Cell Biology 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences carbohydrates (lipids) Isoelectric point chemistry Chemical engineering Surface modification Nanocarriers 0210 nano-technology Biotechnology |
| Zdroj: | Applied Nanoscience. 8:1197-1203 |
| ISSN: | 2190-5517 2190-5509 |
| DOI: | 10.1007/s13204-018-0779-8 |
| Popis: | Chitosan nanoparticles have a huge potential as nanocarriers for environmental and biomedical purposes. Protein encapsulation in nano-sized chitosan provides protection against inactivation, proteolysis, and other alterations due to environmental conditions, as well as the possibility to be targeted to specific tissues by ligand functionalization. In this work, we demonstrate that the chemical modification of the protein surface enhances the protein loading in chitosan nanocarriers. Encapsulation of green fluorescent protein and the cytochrome P450 was studied. The increase of electrostatic interactions between the free amino groups of chitosan and the increased number of free carboxylic groups in the protein surface enhance the protein loading, protein retention, and, thus, the enzymatic activity of chitosan nanoparticles. The chemical modification of protein surface with malonic acid moieties reduced drastically the protein isoelectric point increasing the protein interaction with the polycationic biomaterial and chitosan. The chemical modification of protein does not alter the morphology of chitosan nanoparticles that showed an average diameter of 18 nm, spheroidal in shape, and smooth surfaced. The strategy of chemical modification of protein surface, shown here, is a simple and efficient technique to enhance the protein loading in chitosan nanoparticles. This technique could be used for other nanoparticles based on polycationic or polyanionic materials. The increase of protein loading improves, doubtless, the performance of protein-loaded chitosan nanoparticles for biotechnological and biomedical applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink