Determination of Aflatoxins B1, B2, G1, and G2 and Ochratoxin A in Ginseng and Ginger by Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation: Collaborative Study

Autor: Carolyn J Oles, Joseph M. Betz, Carol M. Weaver, Gregory O. Noonan, Jeanne I. Rader, Frederick S. Fry, Mary W Trucksess
Rok vydání: 2008
Předmět:
Zdroj: Journal of AOAC INTERNATIONAL. 91:511-523
ISSN: 1944-7922
1060-3271
DOI: 10.1093/jaoac/91.3.511
Popis: Botanical products are in great demand in health food markets. Botanicals are used for treatment of diseases as well as to promote health (1). Raw materials for medicinal use and herbal supplements frequently are contaminated with toxigenic fungi originating in the soil; the plants themselves are susceptible to fungal growth both pre- and post-havest under the right environmental conditions (2). Contamination with mycotoxins produced by these fungi could pose human health problems. In recent years, many incidences of natural occurrence of mycotoxins in botanicals have been reported (3). Aflatoxins (AF), the naturally occurring aflatoxins B1, B2, G1, and G2 (AFB1, AFB2, AFG1, AFG2), and ochratoxin A (OTA) have been found in ginseng, ginger, licorice, turmeric, and kava-kava in the United States (4, 5). Fumonisins have been found in medicinal wild plants in South Africa (6) and medicinal plants in Turkey (7) and Portugal (8). Zearalenone was identified in ginseng root (9). The presence of AF and OTA in botanicals may pose risks to human health. AF and OTA were found to co-occur in ginger (5). A multitoxin method for the determination of AF and OTA in botanical roots is needed. A method that is capable of determining AF and OTA simultaneously in powdered ginger and ginseng had been developed (5). The method uses a single immunoaffinity column for cleanup, high-performance liquid chromatography (LC) with post-column derivatization for AF fluorescence enhancement, and fluorescence determination for the toxins. The purpose of this collaborative study was to establish the accuracy, repeatability, and reproducibility parameters of the method by the analysis of spiked powdered ginseng and ginger and naturally contaminated powdered ginger.
Databáze: OpenAIRE
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