Characteristics of regulation of the activity of glucokinase from rat liver
| Autor: | N. Yu. Goncharova, L. Ya. Khu, Alexander M. Rubtsov |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Reaction mechanism Hexokinase biology Glucokinase Glucokinase activity Substrate (chemistry) General Biochemistry Genetics and Molecular Biology Enzyme assay chemistry.chemical_compound Enzyme chemistry Biochemistry Rat liver biology.protein General Agricultural and Biological Sciences General Environmental Science |
| Zdroj: | Moscow University Biological Sciences Bulletin. 70:12-16 |
| ISSN: | 1934-791X 0096-3925 |
| Popis: | A simple method for isolation of glucokinase from a soluble fraction of rat liver was proposed, making it possible to obtain the enzyme preparation characterized by high specific activity, native cooperative characteristics of the enzyme, and actually complete absence of other molecular forms of hexokinase. Dependence of the enzyme activity on the glucose concentration shows a pronounced sigmoidal behavior: with in the whole range of substrate concentrations at 25 and 2°C, the Hill coefficients are equal to 1.76 and 1.35, correspondingly, while the S0.5 values of glucose are 6.90 and 20 mM, respectively. The role of the kinetic reaction mechanism in the regulation of glucokinase activity in liver is under discussion. |
| Databáze: | OpenAIRE |
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