| Autor: |
S. Birkos, Malek Kamoun, Jane Kearns, S. Bassinger, Thomas M. Williams, J. Yee, George D. Montoya, M. McKeen, Jin Wu, B. B. Griffith, I.S. Gourley |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Tissue Antigens. 55:455-459 |
| ISSN: |
0001-2815 |
| Popis: |
Several methods for low-resolution class I typing of potential bone marrow donors are available. The National Marrow Donor Program (NMDP) has initiated pilot projects for large-scale DNA-based class I typing to initially characterize donors. Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) screening of 3,500 NMDP potential donors suggested the presence of variants of known HLA-B*15 variants in 3 donors. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. Sequencing revealed 3 alleles differing from known HLA-B*15 alleles by nucleotide substitutions resulting in predicted novel HLA-B antigens. The new alleles occur in distinct ethnic groups. These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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