Effect of Aβ1-−40on membrane permeability and intracellular free Ca2+ of nerve cells

Autor: Xiaocui Ma, Kechun Lin, Songqing Nie, Yinlin Sha
Rok vydání: 2003
Předmět:
Zdroj: Chinese Science Bulletin. 48:974-978
ISSN: 1861-9541
1001-6538
DOI: 10.1007/bf03184210
Popis: The effects of soluble and fibrillar Aβ1−40 on membrane permeability and intracellular free Ca2+ of nerve cells were investigated by the laser confocal microscopy. Results indicate that: i) Effects of soluble and fibrillar Aβ1−40 on cell membrane permeability are both concentration-dependent. Soluble Aβ1−40increases membrane permeability only at concentration of 3 μmol/L, while the toxic effect of fibrillar Aβ1−40 is much stronger, its evident effect begins from 1 μmol/L. When its concentration rose to 3 μmol/L, not only the membrane permeability increased, but also the nuclear membrane broke seriously, ii) Both soluble and fibrillar Aβ1−40 at high concentrations increased the intracellular free Ca2+, and the increased amplitudes are concentration-dependent. However, the fibrillar one induces the increase of intracellular Ca2+ much quicker and synchronously. These results indicate that some correlation exists between the neurotoxicity of high concentration soluble and fibrillar Aβ1−40 and the change of physico-chemical properties and intracellular Ca ion imbalance.
Databáze: OpenAIRE
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