| Autor: |
F. Sági, G. Börcsök, Tibor Bartók |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Journal of Liquid Chromatography. 15:777-790 |
| ISSN: |
0148-3919 |
| DOI: |
10.1080/10826079208018834 |
| Popis: |
A fully automated RP-HPLC procedure including precolumn derivatization and sample clean-up was developed for the separation and determination of polyamines in plant tissue. The crude plant extract was derivatized with FMOC-CI during 3 min without a prechromatographic purification step. The sample matrix, the reagent excess and most of the reaction by-products were effectively removed by a simple end-cut technique using column switching. The FMOC-polyamines were separated through an ODS microbore column with good resolution and detected with a fluorescence detector. The detection limit was less than 40 femtomoles for all analysed FMOC-polyamines. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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