Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attachedl -glycine
| Autor: | Elvan Hasanoğlu Özkan, Halit Arslan, Nurşen Sarı, Soner Donmez, Fatma Arslan |
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| Rok vydání: | 2017 |
| Předmět: |
Biomedical Engineering
Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology chemistry.chemical_compound Drug Discovery Glucose oxidase Schiff base biology Process Chemistry and Technology General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Carbon paste electrode chemistry Biochemistry Electrode Glycine biology.protein Molecular Medicine Polystyrene Glutaraldehyde 0210 nano-technology Biosensor Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology and Applied Biochemistry. 64:745-753 |
| ISSN: | 0885-4513 |
| DOI: | 10.1002/bab.1533 |
| Popis: | In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified (4-Formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) with L-Glycine (FMPS-Gly). Polymeric microspheres having Schiff bases were prepared from FMPS, Glycine condensation method. Glucose oxidase enzyme (GOx) was immobilized onto modified carbon paste electrode (MCPE) by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2 (+0.5 V vs.Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50°C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0×10−4 to 1.0×10−2 M, R2 = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3 % relative standard deviation. Also, it had good storage stability. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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