Comparison of the effectiveness of three different growth media for primary isolation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish
| Autor: | Sigridur Gudmundsdottir, Sigurdur Helgason, E. Benediktsdóttir |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Veterinary medicine
biology Inoculation Veterinary (miscellaneous) Broodstock Aquatic Science medicine.disease_cause biology.organism_classification Isolation (microbiology) Incubation period Fishery Activated charcoal visual_art medicine visual_art.visual_art_medium Renibacterium salmoninarum Salmo Charcoal |
| Zdroj: | Journal of Fish Diseases. 14:89-96 |
| ISSN: | 1365-2761 0140-7775 |
| DOI: | 10.1111/j.1365-2761.1991.tb00579.x |
| Popis: | The effectiveness of three different growth media KDM-2, S-KDM and S-KDM-C for primary isolation of Renibacterium salmoninarum was examined over a 14-week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S-KDM supplemented with lamb serum and S-KDM-C supplemented with activated charcoal. The third medium was the non-selective serum supplemented KDM-2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S-KDM, 60% on S-KDM-C and 35% on KDM-2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal. |
| Databáze: | OpenAIRE |
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